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KMID : 1098420130210060444
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Korean Journal of Medicinal Crop Science
2013 Volume.21 No. 6 p.444 ~ p.454
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Comparative Genetic Characteristics of Korean Ginseng using DNA Markers
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Shin Mi-Ran
Jo Ick-Hyun
Chung Jong-Wook
Kim Young-Chang
Lee Seung-Ho
Kim Jang-Uk
Hyun Dong-Yun
Kim Dong-Hwi
Kim Ki-Hong
Moon Ji-Young
Noh Bong-Soo
Kang Sung-Taek
Lee Dong-Jin
Bang Kyong-Hwan
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Abstract
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The development of random amplified polymorphic DNA (RAPD) and expressed sequence tag-derived simple sequence repeats (EST-SSRs) provided a useful tool for investigating Korean ginseng genetic diversity. In this study, 18 polymorphic markers (7 RAPD and 11 EST-SSR) selected to assess the genetic diversity in 31 ginseng accessions (11 Korean ginseng cultivars and 20 breeding lines). In RAPD analysis, a total of 53 unique polymorphic bands were obtained from ginseng accessions and number of amplicons ranged from 4 to 11 with a mean of 7.5 bands. Pair-wise genetic similarity coefficient (Nei) among all pairs of ginseng accessions varied from 0.01 to 0.32, with a mean of 0.11. On the basis of the
resulting data, the 31 ginseng accessions were grouped into six clusters. As a result of EST-SSR analysis, 11 EST-SSR markers detected polymorphisms among the 31 ginseng accessions and revealed 49 alleles with a mean of 4.45 alleles per primer. The polymorphism information content (PIC) value ranged from 0.06 to 0.31, with an average of 0.198. The 31 ginseng accessions were classified into five groups by cluster analysis based on Nei’s genetic distances. Consequently, the results of ginseng-specific RAPD and EST-SSR markers may prove useful for the evaluation of genetic diversity and discrimination of Korean ginseng cultivars and breeding lines.
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KEYWORD
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orean Ginseng, Cultivar, Breeding Line, Genetic Characteristics, DNA Marker, RAPD, EST-SSR
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